FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004842584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757230
Sequences flagged as poor quality0
Sequence length300-14999
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11195514.784807786273655No Hit
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74990.9903199820398031No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT54500.7197284840801341No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT31130.4111036276956803No Hit
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28110.37122142545857933No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26180.345733792903081No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT15650.20667432616246054No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG12490.1649432801130436No Hit
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12240.16164177330533655No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA9110.12030690807284446No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC8940.11806188344360366No Hit
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8070.10657263975278318No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT7760.10247877131122644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT418700.0416.45661
AGCAGTG450550.0383.190282
AGTGGTA394400.0380.918825
GTGGTAT389850.0379.236636
CAGTGGT411250.0371.59974
TGGTATC396250.0368.512027
GGTATCA400500.0362.941078
GCAGTGG438650.0354.84483
GTATCAA422950.0334.708869
GTGTATC56600.0264.126346
AGTGTAT59650.0258.878855
CAGTGTA63850.0251.878884
GCAGTGT74200.0230.02173
TGTATCA65650.0229.591697
GTATCAC37950.0157.051859
AAGCATG38400.0143.025381
CATGGTA25850.0136.243234
AAGCGTG18250.0132.252121
ATGGTAT28350.0116.4102555
TAAGCAG33550.0114.517421
Produced by FastQC (version 0.11.5)