FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004842587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996557
Sequences flagged as poor quality0
Sequence length300-15000
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14810414.861568379932105Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85630.8592584267633463No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT82700.8298571983338635Clontech SMART CDS Primer II A (96% over 26bp)
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT42360.425063493608494Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31250.3135796547513088Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28710.2880919004131224No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT23050.23129635334456533Clontech SMART CDS Primer II A (100% over 26bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18050.18112360858435594No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG15190.15242479858151614Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT11530.11569834941704288No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT11470.11509627647992038Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA11110.11148383885718528Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)