FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004842590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925092
Sequences flagged as poor quality0
Sequence length300-14996
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12599613.619834567805148Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84890.9176384619043295No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT67700.7318191055592308Clontech SMART CDS Primer II A (96% over 26bp)
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT41250.4459015968141547Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32000.3459115417709806No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28240.30526693561289037Clontech SMART CDS Primer II A (100% over 25bp)
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT20440.22095099730621387Clontech SMART CDS Primer II A (100% over 26bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17440.18852179026518442No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG12610.13631076692912705Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT10280.11112408279392752No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA10100.10917833037146576Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.107989259446628Clontech SMART CDS Primer II A (100% over 24bp)
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT9580.10355726781768732Clontech Universal Primer Mix Long (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)