FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004842591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002249
Sequences flagged as poor quality0
Sequence length300-14995
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13614413.583849921526486Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86870.8667506777257947No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT76780.7660770926187005Clontech SMART CDS Primer II A (96% over 26bp)
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT40740.40648581340565065Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32710.3263660028595688No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30640.30571245269389147Clontech SMART CDS Primer II A (100% over 25bp)
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT20590.20543797000545772Clontech SMART CDS Primer II A (100% over 26bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19700.19655794119026307No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG13510.13479684190256114Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT11850.11823409152815319No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT11470.11444261855087906Clontech Universal Primer Mix Long (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)