FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004842593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004842593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881128
Sequences flagged as poor quality0
Sequence length300-14993
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11463713.01025503672565Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT68680.7794554253184555Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67710.7684468090901663No Hit
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT39540.4487429749139739Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27780.31527768950708634Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27640.31368881706176627No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT20020.2272087596807728Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG18460.20950418100434895Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.20757483589217457Clontech SMART CDS Primer II A (100% over 24bp)
AAGCATGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13800.15661742675298027No Hit
AAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT10450.11859797895424956No Hit
AAGCAGTGGTATCAACGCAGATACTTTTTTTTTTTTTTTTTTTTTTTTTT9410.10679492650330033Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA9230.10475209050217447Clontech SMART CDS Primer II A (100% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.10270925450104865Clontech SMART CDS Primer II A (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)