FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004850987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004850987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21933279
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT832320.3794781436920581No Hit
CATGAATCCACTCCTGATCCTTACCTTTGTGGCAGCTGCTCTTGCTGCCC731340.3334385159647128No Hit
ATCCACTCCTGATCCTTACCTTTGTGGCAGCTGCTCTTGCTGCCCCCTTT492920.22473611902716414No Hit
CGGCGCCAGAGCTCGCAGTGTTGCCCCAGCCAGAGATGAGGCACTTCGTG455910.2078622170447018No Hit
CTGATCCTTACCTTTGTGGCAGCTGCTCTTGCTGCCCCCTTTGATGATGA389180.1774381295199865No Hit
GTCCCACTCACCATGGAGAAGATCCTGATCCTCCTGCTTGTCGCCCTCTC372440.1698058917683945No Hit
CAGCTGTCCCACTCACCATGGAGAAGATCCTGATCCTCCTGCTTGTCGCC302020.13769942925542505No Hit
GCGGCACCATGGCTTTCCTCTGGCTCCTCTCCTGCTGGGCCCTCCTGGGT279370.12737265595353983No Hit
CATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTGTTGCTGCCC264210.12046078472808375No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC253160.11542277832694327No Hit
CCACTCCTGATCCTTACCTTTGTGGCAGCTGCTCTTGCTGCCCCCTTTGA249050.11354891350262768No Hit
GGGACTTGTAGCAGTGGCCTGCTGATACCACCCACTGTTCGTTGATGAGG248910.11348508355727385No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG241530.11012033358076556No Hit
CCGGGAGATGCGCTGGAGCAGGGGAGAGATGCCCCTGCGCCTAGTGGCCT238780.10886653108274416No Hit
CTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCGATGTTGT227700.10381484683617073No Hit
GTCGGCGCCAGAGCTCGCAGTGTTGCCCCAGCCAGAGATGAGGCACTTCG226390.10321758091893145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCTA57300.066.217364
AATCTAC62900.060.3220185
AATCCAC272800.044.1009525
ATCTACT96050.041.825666
TCTACTT94750.040.0439957
GAATCCA351300.039.595414
CGGAACT84900.033.0964051
CACTCCT369800.031.9693669
TGCCACG90500.030.0710937
TGAATCC408900.029.1773173
ATGAATC543850.029.170262
GCGGTCT77650.025.5161511
CTACTTC157950.024.8886178
TGTCCCA197600.024.266285
ATCCACT539700.023.6383556
CGGTTTT164050.023.099982
ACTCCTG330850.023.0016885
CCACTCA239800.022.9082164
TCCACTC606450.022.496777
ATGCGCT171950.021.689798
Produced by FastQC (version 0.11.5)