FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21949013
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT758990.34579687022828776No Hit
CTGATCCTTACCTTTGTGGCAGCTGCTCTTGCTGCCCCCTTTGATGATGA370500.16880030095202914No Hit
CTGATCCTTACCTTTGTTGCAGCTGCTGTTGCTGCCCCCTTTGATGATGA306530.1396554824583684No Hit
CGGCGCCAGAGCTCGCAGTGTTGCCCCAGCCAGAGATGAGGCACTTCGTG301230.13724079529225303No Hit
CATGAATCCACTCCTGATCCTTACCTTTGTGGCAGCTGCTCTTGCTGCCC235530.10730778645946402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACC316100.034.0048566
GAATCTA82900.033.262954
GATCCTT340500.032.480113
AATCTAC87350.031.733235
TACCTTT356100.030.3683649
CTTACCT387200.028.1325577
TCCTTAC391800.027.9683445
ATCCTTA397950.027.319014
ATCTACT115850.027.2818666
TTACCTT415000.026.2837878
TGCCACG89000.025.319786
TGATCCT469100.024.0367182
CTGATCC541300.021.0448991
TCTACTT141300.020.737577
TCGGAGT101400.020.5345972
GAATCCA304000.020.154524
AGTGTCG107300.019.9950357
CGGAACT66000.019.9783761
AATCCAC251600.018.9150435
CGGTTTT85750.018.0646932
Produced by FastQC (version 0.11.5)