FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19339917
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475040.24562670046619126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT107750.013.5175391
GCGGGTT55400.012.4946771
ACGGTTA20950.012.0297463
CGGGGTA62400.011.3241341
GTCGGTT49350.011.2503781
GGCGGTT58400.010.9885581
CGGGGAT77400.010.8995471
GCCGGTT60550.09.5266311
CGGGTTC99700.09.4656322
CGGGGTG161400.09.2029541
GTCCGGT35300.09.1918161
CGCGGGT52550.08.9187551
CCGGGTT89650.08.839262
ACGGGTT47350.08.6799741
GGCGGTA53300.08.6579751
GGCGGAT111750.08.64611151
GCATTCG43650.08.5781093
CCGGGTA50700.08.5254412
CGGGTGT93150.08.5147791
GCGGATA32200.08.27788452
Produced by FastQC (version 0.11.5)