FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23350468
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780390.7624643754463508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT14750.021.1609081
ACGGTTA40100.019.7507863
CGGTATT33950.016.1179542
CACGGTT51850.015.6915482
CGTATGG34400.015.2763525
CCGGTAT27700.014.4125481
GACGCTA40500.014.222254
CGGGGTT125550.013.9334341
ACGCTAC43250.013.3155495
GTCGAAT26750.013.2962791
CCGTATG39950.013.156474
GCCGGTT59200.012.9969661
CCGTCAT95350.012.789291
GCGGGTT60050.012.5712421
CGGGGAT79100.012.2966321
ACCGTAT44400.012.1622633
CGGGTAT28000.011.924981
TACCGTA52600.011.2223629
TGCGTCG29150.011.1129689
GGCGATT72500.011.0131751
Produced by FastQC (version 0.11.5)