FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22864715
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437700.19143033272008858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT121000.016.6736951
GGCGATT49050.012.9271521
GCGGTAT31700.012.7288151
GCGGGTT53650.011.5501571
GGCGGTT67900.010.3995721
CGGTTAG33500.010.3234813
CGGTATT37500.09.7987042
GTCCGGT48550.09.7952131
CGCGGGT66250.09.7884921
GGCGTTT72650.09.6204471
CTCGGGT80550.09.481991
CGCGGAT38400.09.3820541
CGGGGAT96650.09.2444061
CGGGCAT108450.09.102281
GGCGAAT46150.09.0555631
GTCGGTT45450.09.0364981
CGGGTCT109950.08.9774923
GTCGGAT44350.08.9356941
CGGCATA29850.08.9307332
GCCGATT39750.08.8821511
Produced by FastQC (version 0.11.5)