FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18595013
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT874380.4702228495349801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTA38750.029.1732224
CCGTCAT65900.027.3585111
ACGCTAC44250.025.8699025
CGACCGT13050.022.104841
TACTCTA81100.022.1048249
CGTCATC84950.021.1131232
CGCTACT59000.019.28046
CGCGGGT33650.017.3595261
ACGGTTA33850.017.2362523
CGCAAAT26050.015.5030871
TCATCTA124950.015.2709514
GCATTTA147450.015.1870024
AAGACGC80250.015.0792862
ATCTACT153350.014.2255736
CTAGCAT161050.013.747241
TTAACCC85500.013.6414277
GTAAAAC147750.013.569231
ACCGTTG23800.012.71126653
TACCATC208750.012.5195519
CGCAAGT35300.012.4471992
Produced by FastQC (version 0.11.5)