FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51341732
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG113600.013.944303545
ACCCGGC92300.013.4070975
GGCATAG117550.013.16974135
CATAGGG124700.012.52219137
ATAGGGG131650.012.18559438
GCATAGG135000.011.61694136
ATAGCTC102600.011.55670539
GAGCTTA143950.010.97087544
GTTGTCG25550.010.74548526
CACCCGG118500.010.6902624
ACACGGA116650.010.47205116
CGGACAG119350.010.42415719
CGGACAC119850.010.30507113
TAGCTCT116550.010.23135440
CCGGACA131000.010.06335912
TAGCGTA11450.010.02194437
TGGCATA160700.09.96976334
TCACCCG133600.09.886823
TCAGACG92000.09.8326452
ACGCGCG28200.09.81359420
Produced by FastQC (version 0.11.5)