FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55675015
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG610940.10973324389764422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG179700.015.90282145
GGCATAG194100.014.80191635
ATAGGGG208150.013.73945638
GAGCTTA211850.013.53318144
CATAGGG210650.013.50344337
GTCACTC297400.013.1479338
GGTCACT300900.013.0695537
GCATAGG221150.012.89275936
ACCCGGC104800.012.7806035
TGGCATA230300.012.53982334
AGGTCAC324550.012.3177516
ACTCTCC333750.012.01271311
ATAGCTC110350.011.45649639
GAGGTCA363850.011.2721525
GGGAGGT386800.011.2022393
TAGGGGA349600.011.0871139
CGGACAC118850.011.00615913
GGAGGTC370300.010.9912894
ACGGACA126550.010.869946518
CGTAGGG116500.010.8509440
Produced by FastQC (version 0.11.5)