FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48251879
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG799610.16571582632046308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT98150.022.575086
AACGCTA255500.022.49854923
ACGCTAC257800.022.33314924
TCACGCT270500.021.9375538
GACCGCG100650.021.9285625
TTCGACG25000.021.685056
AGACCGC102200.021.6410034
CGCTACC268200.021.4262825
AAAACGC272400.021.19053621
CCGCGTT107600.020.6133047
GCCGGCT213050.020.5826971
TTCACGC291900.020.468077
ACGCTCA295950.020.28659210
GAAAACG285250.020.22780220
CAGACCG111700.020.0220243
CGCGTTC111700.019.8565798
CACGCTC301250.019.7430089
AAACGCT293350.019.73967622
CCGGCTT250250.019.1235332
CGTGGTT331000.017.4725641
Produced by FastQC (version 0.11.5)