FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55164111
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG670200.1214920331082649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27050.022.2045786
GCCGGCT226750.017.2661761
TCGACGA35750.016.8011287
CCGGCTT259900.016.6798462
CGACGAG35350.016.6731598
ACGCTAC234350.016.2560924
AACGCTA235450.016.15147223
TCACGCT251150.015.8273938
CGCTACC243200.015.67384525
TTCACGC263900.015.25867757
CTACACG89600.015.20546645
GTCCGTT40700.014.8885221
AAAACGC260900.014.710381521
CGGCTTC305700.014.6297723
CCCGTAT36250.014.6033871
TACGGTA84500.014.48318144
ACGCTCA279300.014.417438510
ACGGTAC84300.014.34194445
CACGCTC285300.014.0274979
CCTTACG88200.013.95269941
Produced by FastQC (version 0.11.5)