FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51513355
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT44750.018.5142631
ACGTTCG23450.016.9830786
CCCGTAT31800.015.858881
ACGGTAC74950.015.48995445
AACGCTA76100.015.40373223
TACGGTA75700.015.39593144
CGATTAG31600.015.0238544
TGCGCGT28350.014.999639545
ACGCTAC78050.014.9900624
TCGTATG37900.014.60389943
CCTTACG80850.014.49875241
ATGCGCG29750.014.36940344
CGTGCGA26100.014.31002938
GGGTCTA111500.013.9844162
CGCTACC83650.013.905848525
CGACTTA79200.013.66447138
GTCGGTT39000.013.5660961
AACCGAC76150.013.29580535
CTTACGG90400.013.01685842
TTACGGT91100.013.01562943
Produced by FastQC (version 0.11.5)