FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53758148
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC112700.014.8683265
AGCTTAG146750.014.71078145
GGCATAG159650.013.57920835
ACGGACA126800.013.2891618
CGGACAC127100.013.20272113
TCACCCG136900.012.8803833
CATAGGG171500.012.74300137
GTCACTC234000.012.3995218
CGGACAG134550.012.37338119
ATAGCTC132850.012.29402139
ATAGGGG182400.012.18213738
GAGCTTA179150.012.16419444
CACCCGG140100.012.1532264
GCATAGG179750.012.12048736
CCGGACA142700.011.99536112
ACACGGA138750.011.96642816
CACGGAC143100.011.90114417
GGTCACT243450.011.7435437
TAGCGTA13300.011.68857637
GACACGG142200.011.58051715
Produced by FastQC (version 0.11.5)