FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50601409
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32550.026.40476
TCGACGA38950.022.2971177
CGACGAG38200.021.8514088
AGACCGC106700.020.9599784
CGGTATA55050.020.6805520
CAGACCG116700.019.4723953
CCCGTAT30300.017.1765331
GTTCGAC49900.017.0435525
GACCGCG132400.016.925455
CCCGGAT138950.016.1173651
ACCGCGT141100.015.929696
ATGACGG71300.015.6516816
ACGCTAC172950.015.285789524
AACGCTA174550.015.23584423
TCACGCT184950.015.1333458
GCCGGCT175550.015.1057341
GAACGAT146950.014.92807845
CGGATAC80250.014.8313083
CCGCGTT153500.014.6135477
CGCTACC180500.014.60901725
Produced by FastQC (version 0.11.5)