FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53117921
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG928100.17472445881306237No Hit
GGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACAG549420.10343401805955471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG22100.022.1908536
AACGCTA241150.020.94759423
ACGCTAC241650.020.83026924
AGACCGC95750.020.3013064
CGCTACC247450.020.26037625
GCCGGCT249250.019.6511081
CCGGCTT275600.019.510472
TCACGCT272150.019.235258
ACCGCGT101150.019.1712826
GACCGCG102100.019.0601145
TTCACGC278550.018.9548237
AAAACGC268450.018.90929821
CAGACCG106600.018.2349933
GAAAACG278750.018.1847120
AAACGCT280750.018.0971122
CGGTATA35900.017.92386420
TCGACGA29550.017.5858867
ACGCTCA302950.017.54698810
CGTGGTT297700.017.13376441
GTTTTCG296650.017.1102445
Produced by FastQC (version 0.11.5)