FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48438593
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT557730.11514165987438983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC118150.023.955924
ACCGCGT120250.023.2756166
CAGACCG130250.022.0068363
GACCGCG128600.021.6768535
CCGCGTT135500.020.7224547
GAACGAT138250.020.35907745
CGCGTTC143000.019.6356128
TGCGCGT50900.018.6535145
ATACGGA153200.018.03491233
TTCGACG18400.017.8525776
AATACGG154900.017.85150732
TACGGAG157350.017.41626234
AGAACGA164250.017.2048244
TCAGACC173050.017.1880152
ACGTTCG32650.017.0896996
ATGCGCG56900.016.84469844
GCGTTCT170050.016.6180069
TTCGGCG101450.016.3892244
TCGGCGC100650.016.31830445
CGACGTC59050.015.4693523
Produced by FastQC (version 0.11.5)