FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48438593
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC117550.017.0734315
TCACCCG126100.016.470543
AGCTTAG156450.016.09109545
ACGGACA134450.015.62838218
CGGACAG129900.015.37911319
ACACGGA132000.015.13418116
GGCATAG167600.014.98162435
GTCACTC234650.014.7272748
GACACGG137200.014.5447115
CACGGAC137000.014.49973417
CACCCGG145400.014.2214574
TAGCGTA15700.013.90090437
GAGCTTA184750.013.73802744
GGACACG146900.013.69191614
ATAGCTC141300.013.64751439
CGGACAC153100.013.40198513
AGGTCAC270300.013.3591266
CATAGGG190100.013.30317137
GGTCACT265000.013.1338927
TGGCATA193200.013.11293834
Produced by FastQC (version 0.11.5)