FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51133760
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1626060.3180012578773789No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1339560.2619717384366024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37250.025.6100436
GACCGCG384500.022.2653265
ACCGCGT386900.022.1504736
AGACCGC392450.021.963354
CAGACCG400950.021.5876883
CCGCGTT398550.021.5085537
CGGTATA55800.021.04890820
CGCGTTC410600.020.8658898
GAACGAT408950.020.80228445
TCGACGA46450.020.6344537
TCAGACC447300.019.8701342
CGACGAG47750.019.6481448
GTTCGAC49500.019.4085665
TCACGCT416250.018.8368138
CCGGCTT414850.018.7938062
GCGTTCT461100.018.669919
GCCGGCT377400.018.6189441
CTCAGAC524250.018.2806361
TTCACGC437100.018.1291547
AACGCTA430250.017.8069523
Produced by FastQC (version 0.11.5)