FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46952360
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1496720.3187741787633252No Hit
GCCAGACTCAGAAAAAAGGTGAAATTTAAAACGAACGAGAAGGGGAAGGGG1268390.27014403535839304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA722460.1538708597395317No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA631270.13444904579876282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG47600.027.2738326
GCCGGCT362750.025.903541
CCGGCTT416200.025.0250192
GTTCGAC54300.023.6599375
TCACGCT435150.023.1382968
TTCACGC439350.023.0092837
TCGTATG32200.022.50025745
CGGCTTC476700.022.0608963
CGGTATA82950.021.72668320
TCGACGA62400.021.4901267
ACGCTCA471000.021.30547710
CACGCTC498000.020.3446359
ACCGCGT65200.020.222156
GACCGCG66700.020.0035085
GAAAACG435050.019.80265820
ACGCTAC457150.019.42118324
AGACCGC68100.019.3280644
AACGCTA459900.019.30505223
ATGACGG94600.019.24131216
CGCTACC465400.019.0237325
Produced by FastQC (version 0.11.5)