FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48004929
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG732840.15265932379568772No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA571710.11909402053276653No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG504320.10505587874111845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30550.018.1053426
AGCTTAG233400.015.90034245
GGCATAG248300.014.94377635
GGGAGGT362200.014.936843
GGAGGTC348750.014.6376054
ATAGGGG268350.014.11889338
TGGCATA270400.013.82410834
ACTCTCC362600.013.80554811
GAGCTTA272900.013.68990644
ACCCGGC76850.013.5830385
TGTCGTT42250.013.25602428
GGTCACT380950.013.164357
GTCACTC386250.013.1115218
GCTAAGT44100.013.03044914
ATATAGC49300.012.9772086
TGGGAGG313050.012.7641912
GTCGTTC44600.012.65893429
CATAGGG297400.012.61083537
GCATAGG307900.012.18117736
TCAGACG97900.011.71723652
Produced by FastQC (version 0.11.5)