FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44355238
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT857450.1933142597498857No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC827800.186629592653747No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG818370.18450357542890425No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT636000.14338780010604385No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG497020.1120544094476508No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGA480560.10834346103610132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT363150.017.0717353
GGTCACT352900.017.0132667
GTCACTC354550.016.9391988
TCAGACG149000.016.2623942
GGAGGTC380750.016.0983454
AGGTCAC379500.016.0877486
GAGGTCA390500.015.8905615
ACTCTCC393900.015.32475711
GGCATAG286750.014.55164135
AGCTTAG288550.014.52077745
ATAGGGG295400.014.26107438
TCACTCT425350.014.2149099
CGTCGAT53900.014.02681712
TGGGAGG321600.013.9346342
TGGCATA304050.013.79862134
GCGTCGA54900.013.692883511
CACTCTC441100.013.58413510
CCGTACG28150.013.4389064
TGCGTCG57100.013.32421310
GAGCTTA318150.013.20545244
Produced by FastQC (version 0.11.5)