FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51255622
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG524030.10223854077899981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC166350.022.8860974
ACCGCGT169550.022.4141926
GACCGCG174050.021.8736135
CAGACCG177250.021.415393
CCGCGTT180450.021.07277
CGCGTTC185150.020.5493058
AACGCTA161850.020.08811423
ACGCTAC163600.019.91437724
CGCTACC141800.019.59617825
TCAGACC220300.017.6312032
AAAACGC187300.017.50266321
AATACGG219750.017.17037432
GAACGAT219400.016.99276445
TCACGCT201400.016.9138978
TACGGAG224750.016.76824834
ATACGGA226400.016.75537533
GAAAACG198000.016.62499420
GCGTTCT230250.016.612039
AAACGCT202600.016.29180522
CTCAGAC282700.015.9841581
Produced by FastQC (version 0.11.5)