FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45608614
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG469760.10299808715958789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG146900.016.06270445
GGCATAG162900.014.516170535
ATAGGGG171000.013.96540638
GGTCACT246700.013.2614917
GAGCTTA181750.013.21981444
GTCACTC247750.013.18703658
CATAGGG187850.012.67677437
GGGAGGT289650.012.59323
TGGCATA191600.012.43477834
GGAGGTC277900.012.3235034
ACTCTCC267900.012.25261311
GCATAGG196050.012.211205536
AGGTCAC283950.012.0129246
GAGGTCA325150.010.6364045
TGCCCGT142950.010.4341744
TCACTCT325050.010.2372939
TAGGGGA316350.010.18848139
CACTCTC323050.010.14047310
TAGCGTA11750.09.96092737
TCTCCCC342400.09.71083913
Produced by FastQC (version 0.11.5)