FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61988039
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG655890.10580912230503049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG218050.014.87138145
GGCATAG242450.013.3712235
GTCACTC329000.013.0028878
ATAGGGG254950.012.94519138
GGTCACT327800.012.8513187
AGGTCAC352650.012.3024976
GGGAGGT372250.012.1858493
CATAGGG268450.012.10141537
GAGCTTA270250.012.0722844
GGAGGTC375250.011.7829044
ACTCTCC371150.011.76924411
GAGGTCA383500.011.6998255
GCATAGG279500.011.65509936
TGGCATA289250.011.36334134
TGCCCGT208800.010.937613544
TCAGACG119900.010.5284052
TAGGGGA397800.010.44015539
ACCCGGC95850.010.2121395
TCACTCT436300.010.0479219
CGTCGAT36050.09.988212
Produced by FastQC (version 0.11.5)