FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47196656
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG909620.1927297561081446No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT554840.11755917622638348No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT507150.10745464678684016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG46200.024.4466156
AGACCGC124200.023.0968344
ACCGCGT124300.022.9873856
GACCGCG125550.022.7947225
CGGTATA66300.021.75211520
CGACGAG52650.021.579938
CAGACCG135700.021.3384343
TCGACGA55350.021.0150247
CCGCGTT139050.020.5651347
CGCGTTC145150.019.7783748
TCACGCT299250.018.2019378
GCCGGCT279000.018.1652071
CCGGCTT324950.017.413512
GTTCGAC66050.017.134015
TCAGACC174950.017.0656092
GAACGAT167700.017.04124345
AACGCTA310800.016.96942723
ACGCTAC311350.016.9394524
TTCACGC325700.016.8481037
ACGCTCA331800.016.76890610
Produced by FastQC (version 0.11.5)