FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47196656
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA574420.12170777522882131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG162450.014.08455235
AGCTTAG162800.014.04055645
ATAGGGG172150.013.55218638
GTTGTCG30500.013.20331626
GGGAGGT264150.012.773913
GGTCACT241000.012.7548227
GTCACTC243250.012.6732428
GGAGGTC252900.012.5667454
AGGTCAC256250.012.4616316
CATAGGG188700.012.17291837
TCAGACG89750.012.1398222
ACTCTCC255150.012.12597211
GAGCTTA187750.012.11472244
GAGGTCA268100.012.1142325
TGGCATA190450.012.0966734
AACTCTA114350.011.49004445
GCATAGG203850.011.27927136
TATATAC57250.010.8093087
ATATAGC48000.010.7830836
ACCCGGC68950.010.6416675
Produced by FastQC (version 0.11.5)