FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42956360
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC436930.1017148566591769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG55850.028.316526
TCGACGA60550.026.1185447
CGACGAG63700.024.4738068
CGGTATA90000.024.19873620
GTTCGAC76050.020.558595
ACGAGGA101700.019.42143410
CAGTTCG87900.018.861943
GTATAGA124250.018.70728522
ATGACGG113450.018.38259116
CGAGGAT103650.018.05792411
TATAGAG133650.017.5944623
GACGAGG114800.017.2248239
ATAGAGT135250.017.05483624
AGTTCGA97050.016.5968344
AACGACG33550.016.22760216
TAGAGTT148950.015.8338425
ACTCATT216650.015.58758435
GGTATAG145450.015.31418621
GTTCCTA87650.014.9636515
GTCCGTT33550.014.9054241
Produced by FastQC (version 0.11.5)