FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57667383
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG706930.12258749456343458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34000.021.1103946
TCGACGA40750.017.8338817
GACCGCG95050.017.518185
CGGTATA49500.017.3631320
AGACCGC97200.017.0613884
ACCGCGT98700.016.9149726
AACGCTA251800.015.95858823
ACGCTAC252900.015.86248624
CAGACCG110950.015.4337163
CGCTACC262050.015.23992925
CGACGAG46000.015.1136498
CTACACG92650.014.83766745
AAAACGC282800.014.26492921
TCGTTAC29200.014.10061644
TCACGCT299600.013.9005938
GAAAACG293700.013.72789420
AAACGCT300750.013.47338922
CCCGTAT38750.013.3062051
GTTTTCG307400.013.19658945
TCGTGGT308100.013.03536440
Produced by FastQC (version 0.11.5)