FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57667383
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG609000.10560562458677898No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA578160.10025771413972434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG209250.014.96559845
ATAGGGG219500.014.33900438
GGCATAG218700.014.16537535
GTCACTC277150.013.3475918
GAGCTTA242900.012.93878144
TGGCATA247350.012.65194734
GGTCACT294800.012.5192277
CATAGGG252500.012.36686737
ACTCTCC313450.012.16687411
GCATAGG258750.012.08585536
AGGTCAC328100.011.83239656
GGGAGGT353350.011.7691463
GGAGGTC331550.011.5650814
GAGGTCA348200.011.2512945
GTTGTCG31150.011.12202226
TCACTCT359100.010.6954149
CGTACGC21850.010.2015395
CACTCTC367350.010.15505810
TAGGGGA373150.09.92389539
AGACTCA226200.09.8343914
Produced by FastQC (version 0.11.5)