FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45393599
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG114450.013.8232123
ACCCGGC105700.013.7328725
CCGGACA116450.012.98729912
CGGACAC113550.012.96222813
ACGGACA116850.012.61344418
CACCCGG122650.012.513844
CGGACAG117900.012.21471819
GCGTCGT36800.012.04781911
GACACGG121100.012.02364915
ATAGCTC122350.011.67484639
TCGTGTA36700.011.52871314
ACACGGA124750.011.52712816
CACGGAC126750.011.46891817
TAGCGTA12300.010.60729837
GCCCGGA142850.010.60290510
GTCGTGT43250.010.35514213
TAGCTCT140950.010.1820840
TGATAGC146150.010.0660937
GGACACG146900.09.98883514
GGTCGCC43450.09.88837743
Produced by FastQC (version 0.11.5)