FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46564158
Sequences flagged as poor quality0
Sequence length51-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC263000.026.60324194-95
GATCGGA683900.026.20495294-95
CGTCTGA212550.025.84868294-95
ACGTCTG238250.025.6817594-95
CACACGT298750.025.54056294-95
CACGTCT273150.025.5152894-95
AGATCGG751000.025.16525794-95
CTACACG69650.024.30266856-57
ATCGGAA686200.024.23088594-95
AGAGCAC477300.023.93198694-95
GCACACG356650.023.65290594-95
CGGAAGA594000.023.45108894-95
ACGAGTG60700.023.11286594-95
TAGATCG154900.022.43833594-95
ACCCCTA27200.021.96270670-71
ACTATAG69700.021.5568988-89
GAGCACA497750.021.34851594-95
AAGATCG131000.021.0736394-95
CAGATCG258650.020.9966394-95
AAGAGCA589400.020.1020594-95
Produced by FastQC (version 0.11.5)