FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46564158
Sequences flagged as poor quality0
Sequence length51-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT280350.029.41264394-95
TGTAGGG188850.027.79887894-95
GTCGTGT249450.027.46810294-95
AGCGTCG334550.027.46130894-95
AGAGCGT400750.027.44220294-95
TCGTGTA230000.027.39035694-95
CGTCGTG283700.026.86389594-95
GTAGGGA176000.026.07423294-95
GATCGGA697000.025.49199194-95
AGATCGG753400.024.94123694-95
ATCGGAA661250.024.89907694-95
GTGTAGG222050.024.66157794-95
AAGAGCG488250.024.04440194-95
TCGGAAG639500.023.08498894-95
TTAGGGC59850.022.69039348-49
CGGAAGA667300.020.90247794-95
CAGATCG358550.020.17034394-95
CACAACG81300.019.26140682-83
GAGATCG523000.018.70855394-95
GCTTAGG77050.018.33018546-47
Produced by FastQC (version 0.11.5)