FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51253602
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC94050.022.4321234
CAGACCG100750.021.0970653
GAACGAT109450.019.25434545
GACCGCG115900.018.241975
AATACGG117000.017.84368932
ACCGCGT120000.017.714716
ATACGGA121850.017.28070333
TACGGAG120250.017.19258134
TTCGACG17700.016.6487486
CCGCGTT127800.016.5983287
CGCGTTC132100.016.0580358
AGAACGA132700.015.95239744
AACGCTA81900.015.65647523
ACGCTAC82800.015.35097624
GCGTTCT145100.014.6038399
ACGTTCG18300.014.504896
TTCGGCG86850.014.37574744
CGCTACC88450.014.37040125
CAATACG147900.014.26763831
ACGGAGA147750.014.14486735
Produced by FastQC (version 0.11.5)