FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51253602
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC79500.012.0058855
AGCTTAG87150.011.52144745
ATAGGGG92950.011.08316838
GGCATAG93050.010.97530435
CATAGGG93250.010.9512337
TCAGACG68950.010.7105712
GTCACTC146550.010.5658488
TCACCCG90750.010.518593
GTTGTCG21400.010.51311726
GCATAGG99100.010.21415336
CGTGGCG129000.010.1309451
GCGTAGG43400.010.05484836
ATAGCTC86600.09.89575639
CGTAGGG44650.09.82356437
GAGCTTA103900.09.80704344
GGCGACC132050.09.7009034
GTGGCGA134050.09.5905252
GGCGTAG45750.09.58783635
CACCCGG101500.09.5376354
CCGTACG16650.09.1946024
Produced by FastQC (version 0.11.5)