FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53897770
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG157050.013.79339945
GGCATAG165250.013.08996835
ATAGGGG175200.012.81839138
TCAGACG137850.012.2539262
GAGCTTA183600.011.86395644
CATAGGG191550.011.41567537
GTCACTC264900.011.2740378
GGGAGGT290550.011.0864793
GCATAGG201350.010.99358536
AGGTCAC279950.010.9333846
ACTCTCC282500.010.75522111
TGGCATA201350.010.69348834
GGAGGTC293250.010.6703554
GGTCACT279200.010.5523497
CGTGGCG245400.010.4167411
GAGGTCA306350.010.2135335
GCGTCGA35150.09.96642211
CAGACGT170800.09.6927053
TAGCGTA8200.09.61210937
GTGGCGA263650.09.5593692
Produced by FastQC (version 0.11.5)