FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50018045
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT813860.1627132767784107No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT506700.10130343958865247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT200150.024.7029645
ACCGCGT202200.024.652736
AGACCGC205550.024.2239784
GACCGCG207300.024.0511535
CCGCGTT219300.022.791977
CGCGTTC222650.022.4085858
TTCGACG20300.022.2728846
CAGACCG228700.022.0080383
AGAACGA233100.021.26887144
AATACGG242350.020.34175932
TCAGACC252950.020.1294252
ATACGGA247750.019.93472933
TACGGAG250200.019.66721534
ACGTTCG30850.018.666436
GCGTTCT270700.018.59719
TCGGCGC109500.018.07779345
ACGGAGA274850.017.98478735
CTCAGAC355600.017.269671
CAATACG289300.017.20393231
TCGACGA28350.017.0593247
Produced by FastQC (version 0.11.5)