FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50018045
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG749440.14983392493649042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG213500.017.22829645
GTTGTCG33850.016.74845726
ACCCGGC118350.016.488055
GGCATAG224200.016.35265435
ACGGACA125750.015.55075318
TAGCGTA18700.015.51574437
TCACCCG133900.015.1237893
TGGCATA249250.014.92718634
GAGCTTA253600.014.69829744
GTCACTC343650.014.6393698
GGTCACT340300.014.5626337
ACTCTCC345750.014.48052511
CCGGACA137900.014.12093812
CGGACAC137900.014.02287713
CGGACAG138350.014.00154919
ATAGGGG269100.013.9088638
CATAGGG269900.013.81675737
GGGAGGT409400.013.7401293
GGAGGTC376500.013.5008414
ATAGCTC141250.013.36125439
Produced by FastQC (version 0.11.5)