FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54821431
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG165600.016.17710145
GGCATAG183050.014.52911235
ATAGGGG193050.013.87403538
GAGCTTA198300.013.64581644
GTCACTC259150.013.361678
CATAGGG204600.012.96620937
GGTCACT268600.012.9492967
GCATAGG214500.012.35735836
ACTCTCC292850.012.03384611
AGGTCAC294350.012.01510056
TGGCATA226800.011.82134734
GGGAGGT327600.011.6491183
ACCCGGC95450.011.4426325
TGCCCGT192200.011.42737844
GAGGTCA323350.011.3426065
ATAGCTC87000.011.31496739
GGAGGTC333700.011.139834
TAGCGTA11250.010.40053537
CGTCGAT36100.010.39845412
TCAGACG99150.010.3815192
Produced by FastQC (version 0.11.5)