FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54191875
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG761210.14046570634435512No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT593930.1095976103428789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT128900.024.2290676
GACCGCG132500.023.4520035
AGACCGC134550.023.1615834
CCGCGTT138550.022.5074337
CGCGTTC143400.021.7461998
ACGCTAC198900.021.57223724
AACGCTA199200.021.56238223
CAGACCG150800.020.9643573
CGCTACC205100.020.88731225
AAAACGC229700.018.9049921
CCGGCTT257050.018.4474222
TCACGCT241300.018.4059878
GCCGGCT241250.018.1910611
AAACGCT240000.018.074922
TTCGACG20150.017.9783956
GAAAACG245500.017.74328220
TTCACGC257700.017.4878257
CGTGGTT248250.017.37399741
GAACGAT179700.017.29165545
ACGCTCA270250.016.88385210
Produced by FastQC (version 0.11.5)