FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54191875
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG813050.15003171600908807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC372050.016.4929074
GGCATAG263200.016.39490135
GGGAGGT390250.016.1684553
ATAGGGG269400.016.16774438
AGCTTAG263100.016.10860845
GTTGTCG40950.015.32841326
TGGCATA299050.014.54906534
GAGCTTA300200.014.25323544
ACTCTCC425900.014.03700611
GTCACTC432900.014.0240748
GGTCACT429400.014.0075177
CATAGGG323800.013.33347737
TGGGAGG353400.013.0067112
GAGGTCA488500.012.8460615
GCATAGG343050.012.65088436
ATATAGC58300.012.3898136
CACTCTC491900.012.17206510
CTGGGAG435350.011.9803781
AGGTCAC524000.011.9039266
TCTCCCC504700.011.87306713
Produced by FastQC (version 0.11.5)