FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53816830
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG744410.13832290010392662No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC602800.11200957024038762No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG579490.10767821144426382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG170450.018.40319344
AACGCTA174300.018.27839323
ACGCTAC175550.018.16105724
AGACCGC73600.018.128034
TCGGCGC174650.018.03818745
CGCTACC180900.017.53689425
ACCGCGT77400.017.2090516
GACCGCG79100.016.8391345
CCGTACG193800.016.5089534
CGTACGC196550.016.3352385
GTTAGTT150700.016.1413544
AAAACGC198600.016.09855721
CCCGTAT39250.015.7728051
TCCGTAC204550.015.7512723
TACGCCA205000.015.6619657
GAAAACG204000.015.62830320
CTACACG46350.015.48725345
TTCGACG19450.015.3853786
GTCCGTT40000.015.2519431
AAACGCT212150.015.18700322
Produced by FastQC (version 0.11.5)