FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53816830
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG731600.13594260382857926No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG659160.12248213059000317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG212950.017.13214745
GGCATAG243450.015.25864435
ATAGGGG251300.014.69866138
GTCACTC343450.014.5684068
GGTCACT349550.014.3275017
GAGCTTA258450.014.27642944
GGGAGGT391800.013.8517633
ACTCTCC363900.013.62809311
TGGCATA272450.013.60013634
AGGTCAC381250.013.4774766
GGAGGTC395200.013.1357734
GAGGTCA399900.013.0866565
CATAGGG285450.013.08028837
TGCCCGT250650.013.01003744
TCAGACG135300.012.6509192
TAGCGTA8500.012.44457137
TCACTCT406700.012.441199
GCATAGG303050.012.34108736
CACTCTC422600.011.77612110
TATCCTA114350.011.3138515
Produced by FastQC (version 0.11.5)