FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48642689
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG132200.014.77375745
ATAGGGG144500.013.51494738
GTTGTCG24350.013.21415226
GGCATAG147300.013.04393635
GAGCTTA159450.012.40440744
GGGAGGT231700.012.0416823
CATAGGG166100.011.89135737
GCATAGG174250.011.23190636
TGGCATA173900.011.21706634
GGAGGTC255150.010.5373454
CGGGACG57200.010.18954417
AGCGCTC70000.010.1877989
ACTCTCC259100.010.07970111
ATATAGC40400.09.4649296
CGCTCTC77300.09.312222511
TGTCGTT38250.09.29462528
TCAGACG80050.09.0787022
TAGGGGA289800.08.7418539
GCGCTCT81900.08.73381910
ACGTCTC67600.08.65424821
Produced by FastQC (version 0.11.5)