FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53733463
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT779340.1450381115395447No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG746720.13896740658609702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC189800.020.7333584
GACCGCG200100.019.677655
CAGACCG211700.018.7799453
ACCGCGT212800.018.5344836
TCAGACC262450.015.6721942
CCGCGTT254700.015.4672277
GTCCGTT39100.015.3766921
CCCGTAT36000.015.1996011
AACGCTA130700.014.97582723
CGCGTTC266600.014.8445488
ACGCTAC131750.014.77109324
CTCAGAC317900.014.584591
CGCTACC138300.014.07152225
TTCGGCG81200.013.71543744
TACGGTA83000.013.44510244
ACGTTCG16250.013.291936
ACGGTAC83050.013.27511745
GACGGCA307600.013.17259338
TCGGCGC84450.013.00175845
CGGCAGT314600.012.95101240
Produced by FastQC (version 0.11.5)