FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53733463
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA563370.1048452804912276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG151650.015.00381345
GGCATAG169800.013.47498835
ATAGGGG178200.013.0199638
ACCCGGC90600.012.997945
GAGCTTA183950.012.54077244
CATAGGG185650.012.38676237
GTCACTC240300.012.2311718
GGTCACT240800.012.1583597
TGGCATA195100.011.8481734
TCACCCG105450.011.7864873
GCATAGG195950.011.66692336
ATAGCTC96900.011.59993939
GGGAGGT296950.011.3909243
GTTGTCG25200.011.33201626
AGGTCAC266300.011.2726576
ACTCTCC263400.011.24633811
GGAGGTC285500.010.7062814
CGGACAC115600.010.55966513
CCGGACA118050.010.37748612
TAGCTCT111250.010.26575640
Produced by FastQC (version 0.11.5)