FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54000849
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT706460.13082386908398422No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT677170.12539988028706733No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC640430.11859628355102345No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG623900.1155352205666248No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG611800.11329451505475403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC187350.021.402094
ACCGCGT189050.021.1831636
ACGCTAC336100.020.60591324
AACGCTA336900.020.5501523
GACCGCG195000.020.5494465
CGCTACC219850.020.49969525
GAAAACG279150.020.26282720
CAGACCG200700.020.202713
TCGGCGC165950.020.18709245
CCGCGTT199400.020.061077
CGCGTTC196650.019.6322848
AAAACGC358150.019.4496621
TTCGGCG174250.019.39337744
TCACGCT368650.019.1690778
AAACGCT371000.018.8184522
GTTTTCG371400.018.59136845
TTCACGC386000.018.4881467
GTTAGTT185750.018.4470744
CGTGGTT380550.018.39867841
TCGTGGT379450.018.35714540
Produced by FastQC (version 0.11.5)